234 Microrna-199a-3p Does not Alter Stearoyl-CoA Desaturase Expression in Vitro

Abstract The presence of subcutaneous adipose tissue in excess on a carcass can significantly contribute to financial losses for the producer and processor. Technology with potential limit this accumulation could provide another avenue improve animal efficiency. Stearoyl-CoA desaturase 1 (SCD1) has been identified as genetic target, it seems tohave crucial role fatty acid metabolism. MiR-199a-3p reported alter expression SCD-1, in-vitro, mouse adipocytes; connection yet be formed large mammal adipocytes. Texel-Suffolk (n=5) Suffolk-Suffolk (n=4) cross lambs were finished 203 days age, fat was harvested. Primary pre-adipocytes isolated from both breeds. Pre-adipocytes then propagated, differentiated, treated over 3-day period. Treatments consisted controls, AgomiR-199a-3p (50nm), AntagomiR-199a-3p (500nm). Cells harvested daily 3 determine if day-to-day differences present. upregulated AgomiR treatments compared controls beginning d (P< 0.05). treatment more day comparison 2 downregulated AntagomiR did not differ between non-treatment control Lipofectamine period (P > Data showed no significant alteration SCD-1 or altered using AntagomiRs, but is unaffected. Thus, additional research needed examine targets miR-199a-3p ovine.